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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
13/11/2015 |
Actualizado : |
13/11/2015 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LADO, J.; CRONJE, P.; ALQUÉZAR, B.; PAGE, A.; MANZI, M.; GÓMEZ-CADENAS, A.; STEAD, A.D.; ZACARÍAS, L.; RODRIGO, M.J. |
Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Fruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Physiologia Plantarum, 2015, v.154, no. 4, p. 469-484. |
Serie : |
0031-9317 |
DOI : |
10.1111/ppl.12332 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by
influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase.
Physiol. Plant. MenosABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated diff... Presentar Todo |
Thesagro : |
CITRUS; CITRUS PARADISI. |
Asunto categoría : |
-- |
Marc : |
LEADER 03223naa a2200265 a 4500 001 1053867 005 2015-11-13 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1111/ppl.12332$2DOI 100 1 $aLADO, J. 245 $aFruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit.$h[electronic resource] 260 $c2015 490 $a0031-9317 520 $aABSTRACT. The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase. Physiol. Plant. 650 $aCITRUS 650 $aCITRUS PARADISI 700 1 $aCRONJE, P. 700 1 $aALQUÉZAR, B. 700 1 $aPAGE, A. 700 1 $aMANZI, M. 700 1 $aGÓMEZ-CADENAS, A. 700 1 $aSTEAD, A.D. 700 1 $aZACARÍAS, L. 700 1 $aRODRIGO, M.J. 773 $tPhysiologia Plantarum, 2015$gv.154, no. 4, p. 469-484.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
31/07/2017 |
Actualizado : |
31/07/2017 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
FEITOSA, F. L. B.; OLIVIERI, B. F.; ABOUJAOUDE, C.; PEREIRA, A. S. C.; DE LEMOS, M. V. A.; CHIAIA, H. L. J.; BERTON, M. P.; PERIPOLLI, E.; FERRINHO, A. M.; MUELLER, L. F.; MAZZALI, M. R.; DE ALBUQUERQUE, L. G.; DE OLIVERA, H. N.; TONHATI, H.; ESPIGOLAN, R.; TONUSSI, R. L.; DE OLIVIERA SILVA, R. M.; GORDO, D. G. M.; MAGALHAES, A. F. B.; AGUILAR, I.; BALDI, F. S. B. |
Afiliación : |
FABIELI LOISE BRAGA FEITOSA, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; BIANCA FERREIRA OLIVIERI, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; CAROLYN ABOUJAOUDE, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; ANGÉLICA SIMONE CRAVO, Faculdade de Medicina Veterinária e Zootecnia da USP, Pirassununga, Brazil; MARCOS VINICIUS ANTUNES DE LEMOS, UNESP-Universidade Estadual Paulista, Department of Animal Science, Sao Paulo, Brazil; HERMENEGILDO LUCAS JUSTINO CHIAIA, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; MARIANA PIATTO BERTON, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; ELISA PERIPOLLI, UNESP-Universidade Estadual Paulista, Department of Zootechnics, Sao Paulo, Brazil; ADRIELLE MATHIAS FERRINHO, Universidade de Sao Paulo - USP, Faculdade de Medicina Veterinária e Zootecnia, Sao Paulo, Brazil; LENISE FREITAS MUELLER, Universidade de Sao Paulo - USP, Faculdade de Zootecnia e Engenharia de Alimentos, Sao Paulo, Brazil; MÓNICA ROBERTA MAZALLI, Universidade de Sao Paulo - USP, Food Engineering Department, Sao Paulo, Brazil; LÚCIA GALVAO DE ALBUQUERQUE, UNESP-Universidade Estadual Paulista, Department of Animal Science, Sao Paulo, Brazil; HENRIQUE NUNES DE OLIVERA, UNESP-Universidade Estadual Paulista, Department of Animal Science, Sao Paulo, Brazil; HUMBERTO TONHATI, UNESP-Universidade Estadual Paulista, Department of Zootechnics, Sao Paulo, Brazil; RAFAEL ESPIGOLAN, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; RAFAEL LARA TONUSSI, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; RAFAEL MEDEIROS DE OLIVEIRA SILVA, UNESP-Universidade Estadual Paulista, Department of Animal Science, Sao Paulo, Brazil; DANIEL GUSTAVO MANSAN GORDO, UNESP-Universidade Estadual Paulista, Sao Paulo, Brazil; ANA FRABICIA BRAGA MAGALHAES, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Sao Paulo, Brazil; IGNACIO AGUILAR GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO S. B. BALDI, UNESP-Universidade Estadual Paulista, Sao Paulo, Brazil. |
Título : |
Genetic correlation estimates between beef fatty acid profile with meat and carcass traits in Nellore cattle finished in feedlot. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Journal of Applied Genetics, 2017, 58 (1), 123-132. |
ISSN : |
1234-1983 |
DOI : |
10.1007/s13353-016-0360-7 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 15 December 2015 /Revised: 10 March 2016 /Accepted: 5 July 2016 / Published Online: 30 July 2016 |
Contenido : |
ABSTRACT.
The objective of this study was to estimate the genetic?quantitative relationships between the beef fatty acid profile with the carcass and meat traits of Nellore cattle. A total of 1826 bulls finished in feedlot conditions and slaughtered at 24 months of age on average were used. The following carcass and meat traits were analysed: subcutaneous fat thickness (BF), shear force (SF) and total intramuscular fat (IMF). The fatty acid (FA) profile of the Longissimus thoracis samples was determined. Twenty-five FAs (18 individuals and seven groups of FAs) were selected due to their importance for human health. The animals were genotyped with the BovineHD BeadChip and, after quality control for single nucleotide polymorphisms (SNPs), only 470,007 SNPs from 1556 samples remained. The model included the random genetic additive direct effect, the fixed effect of the contemporary group and the animal?s slaughter age as a covariable. The (co)variances and genetic parameters were estimated using the REML method, considering an animal model (single-step GBLUP). A total of 25 multi-trait analyses, with four traits, were performed considering SF, BF and IMF plus each individual FA. The heritability estimates for individual saturated fatty acids (SFA) varied from 0.06 to 0.65, for monounsaturated fatty acids (MUFA) it varied from 0.02 to 0.14 and for polyunsaturated fatty acids (PUFA) it ranged from 0.05 to 0.68. The heritability estimates for Omega 3, Omega 6, SFA, MUFA and PUFA sum were low to moderate, varying from 0.09 to 0.20. The carcass and meat traits, SF (0.06) and IMF (0.07), had low heritability estimates, while BF (0.17) was moderate. The genetic correlation estimates between SFA sum, MUFA sum and PUFA sum with BF were 0.04, 0.64 and −0.41, respectively. The genetic correlation estimates between SFA sum, MUFA sum and PUFA sum with SF were 0.29, −0.06 and −0.04, respectively. The genetic correlation estimates between SFA sum, MUFA sum and PUFA sum with IMF were 0.24, 0.90 and −0.67, respectively. The selection to improve meat tenderness in Nellore cattle should not change the fatty acid composition in beef, so it is possible to improve this attribute without affecting the nutritional beef quality in zebu breeds. However, selection for increased deposition of subcutaneous fat thickness and especially the percentage of intramuscular fat should lead to changes in the fat composition, highlighting a genetic antagonism between meat nutritional value and acceptability by the consumer.
© 2016, Institute of Plant Genetics, Polish Academy of Sciences, Poznan. MenosABSTRACT.
The objective of this study was to estimate the genetic?quantitative relationships between the beef fatty acid profile with the carcass and meat traits of Nellore cattle. A total of 1826 bulls finished in feedlot conditions and slaughtered at 24 months of age on average were used. The following carcass and meat traits were analysed: subcutaneous fat thickness (BF), shear force (SF) and total intramuscular fat (IMF). The fatty acid (FA) profile of the Longissimus thoracis samples was determined. Twenty-five FAs (18 individuals and seven groups of FAs) were selected due to their importance for human health. The animals were genotyped with the BovineHD BeadChip and, after quality control for single nucleotide polymorphisms (SNPs), only 470,007 SNPs from 1556 samples remained. The model included the random genetic additive direct effect, the fixed effect of the contemporary group and the animal?s slaughter age as a covariable. The (co)variances and genetic parameters were estimated using the REML method, considering an animal model (single-step GBLUP). A total of 25 multi-trait analyses, with four traits, were performed considering SF, BF and IMF plus each individual FA. The heritability estimates for individual saturated fatty acids (SFA) varied from 0.06 to 0.65, for monounsaturated fatty acids (MUFA) it varied from 0.02 to 0.14 and for polyunsaturated fatty acids (PUFA) it ranged from 0.05 to 0.68. The heritability estimates for Omega 3, Omega 6, SFA, MUFA and PUFA... Presentar Todo |
Palabras claves : |
BOS INDICUS; FAT; HUMAN HEALTH; MEAT QUALITY; MEAT TENDERNESS; SELECTION. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 04086naa a2200469 a 4500 001 1057428 005 2017-07-31 008 2017 bl uuuu u00u1 u #d 022 $a1234-1983 024 7 $a10.1007/s13353-016-0360-7$2DOI 100 1 $aFEITOSA, F. L. B. 245 $aGenetic correlation estimates between beef fatty acid profile with meat and carcass traits in Nellore cattle finished in feedlot.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: 15 December 2015 /Revised: 10 March 2016 /Accepted: 5 July 2016 / Published Online: 30 July 2016 520 $aABSTRACT. The objective of this study was to estimate the genetic?quantitative relationships between the beef fatty acid profile with the carcass and meat traits of Nellore cattle. A total of 1826 bulls finished in feedlot conditions and slaughtered at 24 months of age on average were used. The following carcass and meat traits were analysed: subcutaneous fat thickness (BF), shear force (SF) and total intramuscular fat (IMF). The fatty acid (FA) profile of the Longissimus thoracis samples was determined. Twenty-five FAs (18 individuals and seven groups of FAs) were selected due to their importance for human health. The animals were genotyped with the BovineHD BeadChip and, after quality control for single nucleotide polymorphisms (SNPs), only 470,007 SNPs from 1556 samples remained. The model included the random genetic additive direct effect, the fixed effect of the contemporary group and the animal?s slaughter age as a covariable. The (co)variances and genetic parameters were estimated using the REML method, considering an animal model (single-step GBLUP). A total of 25 multi-trait analyses, with four traits, were performed considering SF, BF and IMF plus each individual FA. The heritability estimates for individual saturated fatty acids (SFA) varied from 0.06 to 0.65, for monounsaturated fatty acids (MUFA) it varied from 0.02 to 0.14 and for polyunsaturated fatty acids (PUFA) it ranged from 0.05 to 0.68. The heritability estimates for Omega 3, Omega 6, SFA, MUFA and PUFA sum were low to moderate, varying from 0.09 to 0.20. The carcass and meat traits, SF (0.06) and IMF (0.07), had low heritability estimates, while BF (0.17) was moderate. The genetic correlation estimates between SFA sum, MUFA sum and PUFA sum with BF were 0.04, 0.64 and −0.41, respectively. The genetic correlation estimates between SFA sum, MUFA sum and PUFA sum with SF were 0.29, −0.06 and −0.04, respectively. The genetic correlation estimates between SFA sum, MUFA sum and PUFA sum with IMF were 0.24, 0.90 and −0.67, respectively. The selection to improve meat tenderness in Nellore cattle should not change the fatty acid composition in beef, so it is possible to improve this attribute without affecting the nutritional beef quality in zebu breeds. However, selection for increased deposition of subcutaneous fat thickness and especially the percentage of intramuscular fat should lead to changes in the fat composition, highlighting a genetic antagonism between meat nutritional value and acceptability by the consumer. © 2016, Institute of Plant Genetics, Polish Academy of Sciences, Poznan. 653 $aBOS INDICUS 653 $aFAT 653 $aHUMAN HEALTH 653 $aMEAT QUALITY 653 $aMEAT TENDERNESS 653 $aSELECTION 700 1 $aOLIVIERI, B. F. 700 1 $aABOUJAOUDE, C. 700 1 $aPEREIRA, A. S. C. 700 1 $aDE LEMOS, M. V. A. 700 1 $aCHIAIA, H. L. J. 700 1 $aBERTON, M. P. 700 1 $aPERIPOLLI, E. 700 1 $aFERRINHO, A. M. 700 1 $aMUELLER, L. F. 700 1 $aMAZZALI, M. R. 700 1 $aDE ALBUQUERQUE, L. G. 700 1 $aDE OLIVERA, H. N. 700 1 $aTONHATI, H. 700 1 $aESPIGOLAN, R. 700 1 $aTONUSSI, R. L. 700 1 $aDE OLIVIERA SILVA, R. M. 700 1 $aGORDO, D. G. M. 700 1 $aMAGALHAES, A. F. B. 700 1 $aAGUILAR, I. 700 1 $aBALDI, F. S. B. 773 $tJournal of Applied Genetics, 2017, 58 (1), 123-132.
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